Gene therapy (stem cell and non viral vectors) Essay

Gene therapy (stem cell and non viral vectors) - Essay Example The second approach insists on knocking out or inactivating the muted improperly functioning gene (Al-Dosari, 2009). Lastly, the third approach looks forward to the introduction of a new gene in to the patient’s body to help in fighting the diseases. Chemical vectors in the form of cationic lipids and cationic polymers are known to form condensed complexes with negatively charged DNA via electrostatic forms. Studies have shown that the formed complexes that protect DNA matter and bolster cell uptake coupled with intracellular delivery. Among the most common chemical-based, non-viral vectors are Cationic Lipids (Mukherjee & Thrasher, 2013). Cationic Lipids share similar structures of positively charged hydrophilic, and hydrophobic tails linked via a linker structure. Responsible for the binding of with the cathode phosphate group in the nuclei acids are the positively charged head groups. On the other hand, the hydrophobic tails are typically composed of moieties, cholesterol, and aliphatic chains (Al-Dosari & Gao, 2009). Cationic lipids are mostly applied in the process of liposuction, as such, Al-Dosari & Gao, (2009) coin as the mostly applied gene delivery method. They claim that widely applied co-lipids are cholesterol and DO PE. Transfection tends to be high for lipid composition with high degrees of membrane fluidity. The specified cells particularly well tolerate localized injection. In addition, the method is ideal for DNA-based vaccines development. The method has widely been applied in the direct transfer of skin and thyroid cancer cells for the facilitation of conventional chemotherapy Schlaepfer & Eckel, (2009). Andrà © et al., (2006) showed that following vivo intra-tumoral transfer of short hairpin RNA expression, the total reversal of the phenotype of MDRI and increased efficacy of chemotherapy in thyroid tumor growths inhibition is achievable. Localized injection has demonstrated potential in vivo gene delivery research. For instance,